Decreased Neurofilament L Chain Levels in Cerebrospinal Fluid and Tolerogenic Plasmacytoid Dendritic Cells in Natalizumab-Treated Multiple Sclerosis Patients – Brief Research Report

Moraes, Adriel S. and Boldrini, Vinicius O. and Dionete, Alliny C. and Andrade, Marilia D. and Longhini, Ana Leda F. and Santos, Irene and Lima, Amanda D. R. and Silva, Veronica A. P. G. and Dias Carneiro, Rafael P. C. and Quintiliano, Raphael P. S. and Ferrari, Breno B. and Damasceno, Alfredo and Pradella, Fernando and Farias, Alessandro S. and Tilbery, Charles P. and Domingues, Renan B. and Senne, Carlos and Fernandes, Gustavo B. P. and von Glehn, Felipe and Brandão, Carlos Otavio and Stella, Carla R. A. V. and Santos, Leonilda M. B. (2021) Decreased Neurofilament L Chain Levels in Cerebrospinal Fluid and Tolerogenic Plasmacytoid Dendritic Cells in Natalizumab-Treated Multiple Sclerosis Patients – Brief Research Report. Frontiers in Cellular Neuroscience, 15. ISSN 1662-5102

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Abstract

Background: Neurofilament Light (NfL) chain levels in both cerebrospinal fluid (CSF) and serum have been correlated with the reduction of axonal damage in multiple sclerosis (MS) patients treated with Natalizumab (NTZ). However, little is known about the function of plasmacytoid cells in NTZ-treated MS patients.

Objective: To evaluate CSF NfL, serum levels of soluble-HLA-G (sHLA-G), and eventual tolerogenic behavior of plasmacytoid dendritic cells (pDCs) in MS patients during NTZ treatment.

Methods: CSF NfL and serum sHLA-G levels were measured using an ELISA assay, while pDCs (BDCA-2+) were accessed through flow cytometry analyses.

Results: CSF levels of NfL were significantly reduced during NTZ treatment, while the serum levels of sHLA-G were increased. Moreover, NTZ treatment enhanced tolerogenic (HLA-G+, CD274+, and HLA-DR+) molecules and migratory (CCR7+) functions of pDCs in the peripheral blood.

Conclusion: These findings suggest that NTZ stimulates the production of molecules with immunoregulatory function such as HLA-G and CD274 programmed death-ligand 1 (PD-L1) which may contribute to the reduction of axonal damage represented by the decrease of NfL levels in patients with MS.

Item Type: Article
Subjects: Eprint Open STM Press > Medical Science
Depositing User: Unnamed user with email admin@eprint.openstmpress.com
Date Deposited: 21 Apr 2023 08:10
Last Modified: 25 Sep 2023 05:32
URI: http://library.go4manusub.com/id/eprint/70

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