Application of Derivative Spectrophotometer for Analysis of Chloroquine Phosphate Dosage Forms

Afirst derivative spectrophotometric method was developed for the determination of chloroquine phosphate in formulations.

The aim of the present work is to develop and optimize a derivative spectrophotometric method for the analysis of chloroquine phosphate substance and to study of the expected interference of pharmaceutical excipent used in chloroquine phosphate formulations on the developed method, to employ the developed method for analysis of chloroquine phosphate in pharmaceutical dosage forms, and to compare between the developed method and the official methods for analysis of chloroquine phosphate formulations.

The zero order absorption and first derivative spectra were measured for chloroquine phosphate working standard and formulation using UV spectrophotometer.

The derivative procedure was based on the linear relationship between the concentration of chloroquine phosphate and first order derivative amplitude at 225 nm, 239 nm, 260 nm, and 349 nm. The first derivative spectra were developed between 200 nm- 400 nm. The developed method was compared with high performance liquid chromatography (HPLC) and the official non-aqueous titration methods (BP and USP).

The results obtained showed good linearity and selectivity in the concentration range 2.5 -50 µg/ml.

The developed method was successfully applied in the quantitative assay of commercial tablets, injections and syrup formulations.

The method was simple, rapid and suitable for quality control application.