In Vitro Response of Pigeonpea Mature Cotyledons from various Genotypes on different Culture Media

Efficient plant regeneration via morphogenesis followed by plantlet regeneration has been achieved in pigeonpea (Cajanus cajan L. Mill. sp.). Mature cotyledon explants from 7 days old seedlings produced organogenic callus and somatic embryos, when cultured on MS medium supplement with different concentration and combination of a cytokinin and an auxin. Maximum plantlets were recovered from genotype ICPL-87 on culture medium MS3BN (MS macro and micro salts, vitamins, 30 g l-1 sucrose and 3.0 mg l-1 BAP with 0.5 mg l-1 NAA) mostly via somatic embryogenesis. The rooted plantlets were transferred and acclimatized in glass house where they showed normal morphological characters.