Rapid Micropropagation of Andrographis alata Nees. through Leaf Callus Culture

Nagaraja, Y. P. and Krishna, V. and Maruthi, K. R. (2003) Rapid Micropropagation of Andrographis alata Nees. through Leaf Callus Culture. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 4 (3-4). pp. 117-124.

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Abstract

An efficient protocol for plantlet regeneration through the leaf calli has been established on Andrographis alata Nees. The leaf explants proliferate into luxuriant callus mass on MS medium fortified with 7 �M NAA. Interaction of the same hormones at the concentrations of 1 to 4 �M BAP and 0.5 to 1 �M NAA induced differentiation of shoot buds from the calli. Caulogenesis was optimized (15.05 shoots / callus) at the concentration of 2 �M BAP and 0.5 �M NAA. The microshoots rooted well on MS medium containing 1 �M BAP with 0.75 to 1.25 �M NAA. The plantlets derived from the leaf calli were well acclimatized into soil and exhibited morphological variations when compared to in vivo plants.

Item Type: Article
Subjects: Eprint Open STM Press > Biological Science
Depositing User: Unnamed user with email admin@eprint.openstmpress.com
Date Deposited: 13 Jan 2024 04:39
Last Modified: 13 Jan 2024 04:39
URI: http://library.go4manusub.com/id/eprint/1843

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