ANTIOXIDANT AND CYTOTOXIC EFFECTS OF SILVER NANOPARTICLES SYNTHESISED USING HING OIL

Aim: To find out the antioxidant and cytotoxic effect of Hing oil mediated silver nanoparticles syntheszsed .

Materials and Methods: 0.5mL of hing oil was dissolved in 99.5 mL of distilled water in a conical flask and to this 0.0169gm of silver nitrate was added; the reaction mixture was kept in an orbital shaker with magnetic stirrer and the colour change was noted. Every hour the absorbance was noted using UV-Visible spectroscopy and the final product was centrifuged for 10 minutes and nanoparticles were collected. DPPH assay was carried out with standard procedure to evaluate the antioxidant activity and brine shrimps assay technique to assess the cytotoxic effect.

Results and Discussion: UV-Visible spectroscopy of Hing oil mediated silver nanoparticles showed peak at 420 nm indicating the formation of silver nanoparticles. Transmission electron microscopic images of selenium nanoparticles showed the particle size ranging from 10- 55 nm. The DPPH assay technique showed a dose dependent antioxidant activity of hing oil mediated silver nanoparticles. The antioxidant activity at 10 μl, 20 μl, 30 μl, 40 μl 50 μl was 65.8%, 68.1% 68.5% 69.1% 99.5% respectively. However, cytotoxic activity was not here with the hing oil mediated silver nanoparticle in any of the concentration used.

Conclusion: The present study has concluded that silver nanoparticles synthesized from the Hing oil possess a very good antioxidant effect and it does not have any cytotoxic effect because all the brine shrimps were alive. Hence it is bio-friendly and may be used for beneficial biomedical application.