AMELIORATIONS OF VITAMIN E, OLIVE OIL AND STRAWBERRY FRUIT EXTRACTS AGAINST NAF (SODIUM FLOURIDE) INDUCED HEPATO-HISTOPATHOLOGIES

Hepato-protective properties of strawberry fruit pulp extracts and vitamin E after low dose of (50ppm ad libitum) fluoride exposure were examined through serological and histopathological study in mice. Study comprised of 5 groups i.e. (i) Control (Ct) (Flouride free water for drinking for 15 days); (ii) NaF (F) {50 ppm Sodium Flouride (NaF) was given for first 10 days}; (iii) Sodium Fluoride and vitamin E (FE) {50 NaF for 10 days followed by Fluoride free water plus 60 µg Vitamin E by utilizing corn oil acting as a vehicle for next 5 days}; (iv) Sodium fluoride and Strawberry pulp extract (FS) and (v) Sodium fluoride and Olive Oil (FO) {50 ppm Sodium fluoride for 10 days followed by Fluoride free water plus 0.2 mL strawberry pulp and olive oil respectively (5 days)}. The hepato-histopathological indications of F halogen include varied signatures of apoptosis, enflamed sinusoidal spaces and hepatocytic volume along with nuclear alterations. The micrometric results show a significantly (p=0.05) higher mean CSA of hepatocytes in F (804.5±25.04 µ2) as compared to Ct (429±10.75 µ2) group. The mean breadth of sinusoids in F (2.0±0.13 µ) group remains significantly lower than that of Ct (8.73±0.11 µ) and in other experimental groups e.g. FE (8.19 ± 0.23 µ), FO (7.76 ± 0.28 µ) and FS (7.02 ± 0.23 µ). Similarly sereological estimations showed a significantly (p=0.05) higher bilirubin amount present in F (0.7 ± 0.054 mg/dL) than that of Ct (0.66 ± 0.068 mg/dL) and in other co-treated groups like in FE (0.6 ± 0.044 mg/dL), FS (0.48±0.02 mg/dL) as well as FO (0.46 ± 0.04 mg/dL). Similarly, F (111.8 ± 9.58 µL/L) had highest SGPT value as compared to Ct (49 ± 2.16 µL/L) group. Likewise, SGOT concentration was also highest in F (140.4 ± 11.81 µL/L). Alkaline phosphatase (240.4±2.69 µL/L) and total proteins (7.84±0.12 g/dL) was found high in F group as compared to Ct {(192.6±3.81 µL/L & 5.36±0.24 g/dL respectively)}. Serological results also indicated rehabilitative potentials of vitamin E as well as strawberry fruit pulp in terms of plasma bilirubin SGPT, SGOT and alkaline phosphatase. Based upon these findings it is concluded that vitamin E and strawberry fruit extracts work as the rescuing agent against hepatohistological exposure of fluoride.