Effectiveness Test of Orchid Mycorrhizal Isolate (Ceratorhiza and Trichoderma) Indonesia

Aim: The existence of Orchid Mycorrhizal Fungi (OMF) has a role to stimulate growth and support the
supply of orchid nutrition as a biofertilizer agent. This study aimed to determine the association of
mycorrhizal with Phalaenopsis amabilis (L.) Blume which was carried out through the effectiveness
test of two Indonesian orchid mycorrhizal isolates i.e. Ceratorhiza and Trichoderma.
Study Design: This study consisted of 4 treatments. Each treatment was repeated 3 times, each
repetition of 5 plantlets, so that the total plantlet used was 60.
Place and Duration of Study: Laboratory of Plant Biotechnology, Department of Biology, Universitas
Gadjah Mada, Indonesia, between June 2017 and April 2018.
Methodology: The method of inoculating orchid mycorrhizal by placing a plantlet in a petri
dish containing orchid mycorrhizal for 1, 2, 3 and 4 days. Then plantlets are grown on sterile moss
growing media and acclimatized in a greenhouse. Observation of each treatment is carried
out every day for the next month. Observation variables include the number of initial
and final roots, the number of live and dead roots, and the number of living and dead plants.
Results: The results of the orchid mycorrhizal induction test showed that the Ceratorhiza
inoculation treatment showed a fluctuation in the mean increase in the number of final roots,
live roots, dead roots, and dead plantlets that were higher than the Trichoderma inoculation treatment.
The results also showed that the best inoculation time on Ceratorhiza and Trichoderma was day 3
and 4. The adaptation process had the effect of increasing the number of dead roots in weeks 1 and
2. The adaptation process stopped at the beginning of week 4 with the number of new roots
appearing a lot.
Conclusion: Orchid mycorrhizal Ceratorhiza shows the value of effectiveness test compared with
Trichoderma. The results of this study are expected to be basic information in efforts to cultivate
natural orchids in Indonesia.