THE EFFECTS OF PLANT GROWTH REGULATORS ON PHENOLIC AND FLAVONOID CONTENT IN CALLUS CULTURES OF Ardisia silvestris Pitard

Ardisia species are highly-prized for their medicinal value. Due to their habitat loss and exploitation for medicinal purposes, many species in this genus have become threatened. One of the most important medicinal species in the Ardisia genus is A. silvestris Pitard. The current study evaluated the roles of TDZ combination with different auxins (NAA and IAA) and their concentrations in the accumulation of phenolic and flavonoid content of A. silvestris callus. In research study, the maximum phenolic content was observed in the medium with 1 mg.L-1TDZ supplemented with 0.2 mg.L-1 NAA. Meanwhile, a medium containing 1.0 mg.L-1 TDZ and 0.2 mg.L-1 IAA showed the greatest level of flavonoid content.