Recent Advancement on Preventative Effects of Lactobacillus plantarum YS-3 on Oxazolone-induced BALB/c Colitis in Mice

In this study, the preventative effects of Lactobacillus plantarum YS-3 (LP-YS3) on colitis were studied
using an in vitro animal experiment. Oxazolone was administered to BALB/c mice to induce colitis,
and the preventive effects of LP-YS3 were determined using serum- and tissue-related indexes. The
mice treated with LP-YS3 showed a significant decrease (p < 0.05) in disease activity index for colitis
by inhibiting colon shortening and increasing colon weight/length ratio. The application of LP-YS3
resulted in a significant reduction in myeloperoxidase, nitric oxide, and malondialdehyde activities and
a decrease in glutathione activity (p < 0.05) in mouse colon tissues. LP-YS3 also significantly
increased serum interleukin-2 and reduced interleukin-10 cytokines levels in mice (p < 0.05). Reverse
transcription-poly-merase chain reaction and western blot assays showed that LP-YS3 application
resulted in a significant increase in neuronal nitric oxide synthase, endothelial nitric oxide synthase, c-
Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8,
and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p < 0.05). LP-YS3
significantly decreased the contents of MPO, NO, MDA, and IL-10 in colonic tissues of colitis rats and
increased the content of GSH and the serum level of IL-2. LP-YS3 also increased the expression of
nNOS, eNOS, c-Kit, and SCF and reduced the expres-sion of iNOS, IL-8, and CXCR2 in colonic
tissues, suggesting that LP-YS3 can effectively prevent oxazolone-induced colitis and may be
potentially used for probiotic development. These findings indicate that LP-YS3 imparts preventative
effects on oxazolone-induced colitis in mice.