AMELIORATIVE POTENTIALS OF DICHLOROMETHANE EXTRACT OF Musa acuminata LATUNDAN BRACT IN INDOMETHACIN ADMINISTERED WISTAR RATS

In this study, the ameliorative potentials of dichloromethane extract of Musa acuminata Latundan bract was investigated in Wistar rats administered indomethacin. A single oral dose of 40mg/kg body weight indomethacin was administered to forty five adult male Wistar rats (120-180g) in five groups of nine rats each. The bioactive components of dichloromethane extract of the sample was carried out using gas chromatography-mass spectrometry (GC-MS) methods. Faecal occult blood tests (FOB) of animals were carried out using immunochromatographic sandwich methods. Thereafter, concentrations of 100mg, 150mg and 200mg/kg bodyweights dichloromethane extract of M. acuminata Latundan bract were administered to the animals for three weeks. Effect on some biochemical indices, hematological indices as well as histopathology of kidney, stomach and liver were analysed. GC-MS analyses showed n-Hexadecanoic acid, 9,12-Octadecadienoic acid and 9-Octadecanoic acid as the predominant compounds in the sample analysed. FOB tests were positive (+) at week 0 and week one, then negative (-) at weeks two and three in all treated groups. Serum levels of Aspartate-aminotransferase, Alanine-aminotransferase, Alkaline-phosphatase, creatinine and urea showed a dose-dependent decrease significantly at (p≤ 0.05) in treated groups (66.33+1.00 u/l, 43.66+1.15 u/l, 68.67 +1.15 u/l, 170.00 +1.77 umol/l and 8.11+0.03 mmol/l) compared to the untreated control (72.33+0.42 u/l, 56.33+0.60 u/l, 75.33+1.16 u/l, 194.33+0.60 umol/l and 9.97+0.15 mmol/l) respectively. A significant dose-dependent increase (p≤ 0.05) in packed cell volume, hemoglobin and red blood count was also observed in treated groups when compared to the untreated control. Kidney, stomach and liver tissues showed normal histology in treated groups. Findings from this study revealed that the dichloromethane extract of M. acuminata Latundan showed dose-dependent ameliorative potentials and non-impairment of hepatorenal function in Wistar rats administered indomethacin.