IMPROVEMENT IN ISOLATION AND AXENIC GROWTH OF GIARDIA LAMBLIA IN TYI-S-33 MEDIUM SUPPLEMENTED WITH BILE

total of 60 fresh Giardia lamblia cyst present in human fecal specimens isolated in Central Laboratory of shan University of Medical Sciences. Thirty-eight new isolates of G. lamblia was gradually adapted to as in TYI-S-33 medium supplemented with bile. The parasites grew luxuriantly and formed a monolayer the surface of the culture vessels. When G. lamblia was inoculated into to fresh medium, the population of separasites grew from 0.9 x 105 to 1.6 x 105 /ml in six days with a generation time of 12 to 13 hrs at 37°C.