Production of Synthetic Seed from Encapsulated Nodal Segments of Giloe (Tinospora cordifolia Willd.)

Tinospora cordifolia (Willd.) Miers is generally acknowledged as giloe belong to the family Menispermaceae employed for medicinal purpose, particularly in Ayurveda for curing different diseases.

Standardization of the concentration of encapsulation matrix and hardening solution affecting the frequency of germination to plant idyllic synthetic seeds of Tinospora cordifolia tracked by an efficient plantlet regeneration.

The current investigation was carried out at Plant Tissue Culture & Genetic Transformation Laboratory, Department of Plant Molecular Biology & Biotechnology, College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during 2017 to 2020.

A procedure was recognized for encapsulation of nodal segments of Tinospora cordifolia excised from 18-month-old in vitro derived plants for short-term conservation and further propagation. Conservation strategies prerequisite to be implemented for constant supply to encounter the ever-increasing demands and sustainable utilization of resources. Diverse concentrations and combinations of gelling matrix viz., sodium alginate and complexing agent i.e., Calcium chloride were experienced to optimize amalgamation to achieve uniform beads.

The best gelling composition was established using 2.5% sodium alginate and 100 mM calcium chloride. The maximum conversion rate of encapsulated beads was attained on Murashiage and Skoog’s medium amended with 2.0 mgl-1 BAP in association with 0.2mgl-1 NAA. Well, established regenerants were hardened successfully, acclimatized and established first under net house circumstances tracked under field conditions.

In this research, syn seed production procedure in giloe (Tinospora cordifolia) has been standardized. Current method warrants feasible tactic for multiplication, conservation and germplasm exchange through artificial, seed development.