Ex-vivo Acetylcholinesterase and Butyrylcholinesterase Inhibitory Activities Assay of G. asiatica and G. tiliaefolia (Tiliaceae) Leaves

Aims: Our study was carried out to appraise acetylcholinestrase (AChE) and butyrylcholinestrase (BChE) inhibitory activities of Grewia asiatica and Grewia tiliaefolia leaves extracts.

Study Design: For the purpose of these experiments the extracts were subjected to an ex-vivo study.

Place and Duration of Study: The study was carried out between June 2018 to December 2018 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh.

Methodology: In this study, cholinesterase inhibitory activities of different fractions of crude ethanol extract of both plants were examined using swiss albino mice at 300 mg/kg b.w. dose. We determined anti-acetylcholinestrase (AChE) and anti-butyrylcholinestrase (BChE) activities using slightly modified Elman coupled enzyme assay.

Results: The highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by PEF and CLF of G. asiatica with the IC50 values were found to be 55.88 μg/ml and 26.14 μg/ml respectively whereas the highest inhibition of bovine brain acetylcholinesterase and human blood butyrylcholinesterase were exhibited by CLF of G. tiliaefolia.

Conclusion: The result of the present study on various fractions of these plants has a considerable anti-acetylcholinesterase and anti-butyrylcholinesterase activities which suggest its effectiveness against various neurodegenerative disorders.